Path to raw non-stitched images: LCSB_HCS\YokogawaCV8000Standalone\BTSData\MeasurementData\isabel\IR_20220915_488Ki67_568Edu_647Sox2_RS_e51e52e54d30_20220915_175904\09-15-22_18-04-42 LCSB_HCS\YokogawaCV8000Standalone\BTSData\MeasurementData\isabel\IR_20220915_488Ki67_568Edu_647Sox2_RS_e53d30edu8h_20220919_134031\09-21-22_09-07-27 LCSB_HCS\YokogawaCV8000Standalone\BTSData\MeasurementData\isabel\IR_20230523_568Edu_647Sox2_RS_b55b56b57d30_20230521_183416\05-23-23_10-28-11 Raw stitched images can be found in: LCSB_Cellular_Biology\16-Our Papers\In Preparation\GBA hMO_Isabel\Figures\Supplementary Figures\Fig S9\A\Partials LCSB_Cellular_Biology\16-Our Papers\In Preparation\GBA hMO_Isabel\Figures\Supplementary Figures\Fig S10\A\Partials Barcodes for EdU 8h pulse: IR_20220915_488Ki67_568Edu_647Sox2_RS_e51e52e54d30_20220915_175904 IR_20220915_488Ki67_568Edu_647Sox2_RS_e53d30edu8h_20220919_134031 Barcodes for EdU after 7 days from 8h pulse: IR_20230523_568Edu_647Sox2_RS_b55b56b57d30_20230521_183416 To analyze them, use the image analysis scripts in LCSB_Cellular_CellularBiology\16-Our Papers\In Preparation\GBA hMO_Isabel\Scripts\Image analysis quantification The slidelayouts for each barcode in the Accessory Files folder